人Caspase-3 FlowCytomix Simplex半胱天冬酶检测试剂盒

英文名称：Human Caspase 3 FlowCytomix Simplex
中文名称：人Caspase-3 FlowCytomix Simplex检测试剂盒

Human Caspase 3 FlowCytomix Simplex 产品介绍：
FlowCytomix Simplex试剂盒设计用于在流式细胞仪上免疫测定人Caspase-3 （半胱天冬酶-3）。
与FlowCytomix Human Basic Kit （cat. BMS8420FF）一起，它可用于单独检测Caspase-3或者与其它Simplex kits一起使用检测不同裂解物。

Caspases （半胱天冬酶） 是天冬氨酸特异性半胱氨酸蛋白酶，如激酶一样可以进行级联反应。半胱天冬酶存在于所有细胞中，这些蛋白酶募集形成低聚体可致其激活并伴随自我蛋白酶解。有活性的半胱天冬酶可以激活额外半光天冬酶产生级联反应，切割决定细胞存活的蛋白质。
这种级联信号通路zui终结果是称为程序性细胞死亡的细胞凋亡。半胱天冬酶亚群充当引发器和效应器。Caspase-3特异性切割底物蛋白中天冬氨酸残基羧基 端。活性Caspase-3有两个活性位点，包含两个相同分子量（～20 kDa）大亚基和两个小亚基（～10 kDa），它们来自两个前体Caspase-3多肽。Caspase-3可被其它半胱天冬酶水解激活。 

大小亚基都有助于底物结合和催化。可以与底物共价结合活性位点位于大亚基C末端附近。Caspase-3双重对称，两个活性位点口袋分别位于对称轴 两端。Caspase-3，同caspase 8 and 9都处在细胞凋亡路径关键部位。Caspase-3可以增强caspase 8 and 9起始信号致使细胞凋亡裂解。

 Description: This FlowCytomix Simplex Kit is designed for the measurement of Human Caspase-3 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect Caspase-3 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population B9.

Caspases are a family of aspartate-specific cysteine proteases that act in a step-wise signaling manner like kinases.
Caspases are present in all cells, recruitment of these proteases to oligomerized receptors leads to activation accompanied by autoproteolytic cleavage. Active caspases can proteolyze additional caspases generating a caspase cascade that cleaves proteins critical for cell survival. The final outcome of this signaling pathway is a form of controlled cell death termed apoptosis. The subgroup of caspases involved in apoptosis is called initiators or effectors. Caspase-3 cleaves substrate at the carboxyl terminus of aspartate residues. Active caspase-3 has two active sites and consists of two identical large (~ 20 kDa) and two identical small (~ 10 kDa) subunits that are derived from two precursor caspase-3 polypeptides. Caspase-3 is proteolytically activated by other caspases.

Both subunits contribute to substrate binding and catalysis. The active site cysteine that covalently binds the substrate is located near the C-terminus of the large subunit. Active caspase-3 has two-fold symmetry, two active site pockets each residing on an opposite side. Caspase-3, together with caspases 8 and 9, is situated at pivotal junctions in apoptotic pathways. Caspase-3 appears to amplify caspase 8 and 9 initiation signals into complete commitment to apoptotic disassembly.

参数：
? 反应：人
? 样品体积：25 µL
? 适合样本类型：细胞裂解物
? 灵敏度：20 pg/mL
? 标准曲线范围：82 – 60,000 pg/mL
? 成份：荧光珠1瓶（20倍浓缩），175 µl （涂有人Caspase-3单抗，具有Bead Population B9）；人Caspase-3标准物2瓶（冻干），1.2 µg/ml （重配制）；蛋白生物素结合物1瓶（20倍浓缩），350 µl （人Caspase-3多抗）；1瓶细胞裂解液浓缩物（10倍浓缩），15ml
? 应用：多重免疫检测