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产品名称：干细胞生长分化因子
干细胞生长分化因子的产品信息
Wnt糖蛋白是存在于多种生物体内的一种细胞外配体，Wnt作为形态发生素通过激发细胞内远离信号发送区域的浓度依赖反应控制胚胎形态发育。Wnt通路调节动物发育过程中的多个重要环节，例如细胞增殖、细胞迁徙及细胞分化。Wnt蛋白通过自分泌或旁分泌作用与位于细胞膜上的受体相结合，激活细胞内信号通路调节靶基因的表达，对细胞的增殖、分化、迁移、极性化和凋亡起到重要作用。
WNT-5a,human W5A-H-005,5ug 产品简介
Wnt信号转导通路因其启动蛋白为Wnt蛋白而得名，在胚胎发育、细胞增殖及分化等方面发挥重要 作用，其异常活化与人类多种肿瘤的发生密切相关。研究显示，Wnt信号至少存在经典和非经典两条不同的信号通路，而Wnt-5a属于非经典Wnt信号通 路，既往研究Ror2具有Wnt受体结构域样结构。有研究认为Wnt-5a经由Ror2介导的 信号通路发挥作用。
Human WNT-5a蛋白含有357个氨基酸残基。
人源WNT-5a 蛋白因子，5ug 产品特性
? 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定，纯度高，无动物成分污染
?人源WNT-5a 蛋白因子，5ug 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定
? 人源WNT-5a 蛋白因子，5ug 产品经过SDS-PAGE 和HPLC双层鉴定纯度大于85%
人源WNT-5a 蛋白因子，5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
产品英文描述
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product WNT-5a,human W5A-H-005,5ug is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
?WNT-5a,human W5A-H-005,5ug Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
?WNT-5a,human W5A-H-005,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparat
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