Anti-Ubiquitin （linkage-specific K63） EPR8590-448 antibody 抗泛素抗体

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Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗体产品信息：

 Product name：Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibodySee all Ubiquitin primary antibodies

    Description

    Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)

    Tested applicationsWB, IHC-P, ICC/IF more details

    Species reactivity

    Reacts with: Mouse, Rat, Human

    Positive control

        K63-linked-Ub2-7 recombinant protein; Human kidney carcinoma tissue.

Properties

    FormLiquid

    Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    PurityTissue culture supernatant

    ClonalityMonoclonal

    Clone numberEPR8590-448

    IsotypeIgG

    Research Areas

        Neuroscience

        Neurology process

        Neurodegenerative disease

        Alzheimer's disease

        Tangles & Tau

        Epigenetics and Nuclear Signaling

        Chromatin Modifying Enzymes

        Ubiquitylation

        Epigenetics and Nuclear Signaling

        DNA / RNA

        DNA Damage & Repair

        DNA Damage Response

        Other

        Cell Biology

        Proteolysis / Ubiquitin

        Proteasome / Ubiquitin

        Ubiquitin

        Neuroscience

        Neurology process

        Neurodegenerative disease

        Other

        Epigenetics and Nuclear Signaling

        Ubiquitin & Ubiquitin Like Modifiers

        Ubiquitin like Modifiers

Applications

Our Abpromise guarantee covers the use of ab179434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application      Notes

WB           WB: 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.

IHC-P       IHC-P: 1/100 - 1/250.

ICC/IF      ICC/IF: 1/100 - 1/250.

Application notesIs unsuitable for ,Flow Cyt or IP.

Target

    RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

    Cellular localizationCell Membrane, Cytoplasmic and Nuclear

    Database links

        Entrez Gene: 7314 Human

        Entrez Gene: 22187 Mouse

        Entrez Gene: 192255 Rat

        Omim: 191339 Human

        SwissProt: P0CG47 Human

        SwissProt: P0CG48 Human

        SwissProt: P62988 Human

        SwissProt: P0CG49 Mouse

Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody images

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with ab179434 at 1/100 dilution.

    Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    All lanes : Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody (ab179434) at 1/1000 dilution

    Lane 1 : Ubiquitin recombinant protein

    Lane 2 : K6-linked-Ub2 recombinant protein

    Lane 3 : K11-linked-Ub2 recombinant protein

    Lane 4 : K27-linked-Ub2 recombinant protein

    Lane 5 : K29-linked-Ub2 recombinant protein

    Lane 6 : K33-linked-Ub2 recombinant protein

    Lane 7 : K48-linked-Ub2-7 recombinant protein

    Lane 8 : K63-linked-Ub2-7 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size : 77 kDa

公司介绍：

    Abcam位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司，理念是Abcam销售质量高的抗体，并提供zui全面、诚实、时新的说明书，快速的送货服务，及高效率的顾客和服务。我们的在线目录(www.abcam.cn) 已有差不多100,000种抗体和试剂，并不断添加，供应予百多个国家。Abcam于2005年11月在伦敦证券交易所上市，在美国马萨诸塞州、日本东京及香港均设有分公司。

    Abcam 一直致力加强产品线，为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的MitoSciences公司，加强了免疫分析方面的产品供应；同年也并购了英国的Ascent Scientific 公司，开展了生化试剂的供应。在2012年，Abcam并购了美国的Epitomics 公司，成为一家有的RabMAbs® 供应商。

    Abcam 的目标是给世界上的抗体建立zui大的在线目录，为各地科学家提供*产品，成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结果。为提供高质量的抗体来指向尽可能多的靶蛋白，我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策，在我们的上会发布有关我们每一种产品的尽可能多的信息。

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