Anti-Cdk9 antibody 抗细胞周期依赖性激酶抗体

Anti-Cdk9 antibody 抗细胞周期依赖性激酶抗体

• Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
• Description

  Rabbit polyclonal to Cdk9

• Tested applicationsIP more details
• Species reactivity

  Reacts with: Human
  Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan

• Immunogen

  Synthetic peptide, corresponding to to a region within amino acids 1-50 of Human Cdk9 (NP_001252.1).

• Positive control
  • HeLa whole cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium azide
  Constituent: 99% Tris citrate/phosphate
  Note: pH 7 to 8

• Concentration 100 µg at 1 mg/ml

• PurityImmunogen affinity purified
• Purification notesab124261 was affinity purified using an epitope specific to Cdk9 immobilized on solid support .
• Clonality Polyclonal
• IsotypeIgG

• Applications

  Our Abpromise guarantee covers the use of ab124261 in the following tested applications.

  The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  Application	Notes
  IP	IP: Use at 2-10 µg/mg of lysate.

• Application notesIs unsuitable for or WB.

• Target

• FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

• Alternative names

• C-2K antibody
• CDC2 related kinase antibody
• CDC2L4 antibody

• Anti-Cdk9 antibody images

• 

  Immunoprecipitation - Anti-Cdk9 antibody (ab124261)

  Anti-Cdk9 antibody which recognizes a downstream epitope of Cdk9 was used at 1 µg/ml detecting Cdk9 in HeLa whole cell lysate by WB following IP.
  
  Lane 1: IP with ab124261 at 6 µg/mg of lysate.
  Lane 2: IP with an antibody which recognizes an downstream epitope of Cdk9.
  Lane 3: Control IgG.
  
  In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
  
  Detection: Chemiluminescence with an exposure time of 10 seconds.

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