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Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗体产品信息:
Mouse monoclonal [4C11C10D12] to ATP5O
Reacts with: Cow, Human, African Green Monkey
Recombinant mature Human ATP5O.
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab110276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
ICC/IF | ICC/IF: Use a concentration of 0.5 - 1 µg/ml. heat-induced antigen-retrieval improves signal |
WB | WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa. |
Flow Cyt | Flow Cyt: Use a concentration of 1 µg/ml. |
IHC-P | IHC-P: 1/500. |
Target
Database links
Alternative names
Anti-ATP5O [4C11C10D12] antibody images
Western blot - ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Predicted band size : 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)
Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)
Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)
HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/mlSecondary antibody: Goat-Anti-Mouse-IR800 1:4000
公司介绍:
Abcam位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。主要创立人Jonathan Milner和实验室同事构思成立一间了解科研人员需要的网上抗体公司,理念是Abcam销售质量高的抗体,并提供zui全面、诚实、时新的说明书,快速的送货服务,及高效率的顾客和服务。我们的在线目录(www.jiefang996.com) 已有差不多100,000种抗体和试剂,并不断添加,供应予百多个国家。Abcam于2005年11月在伦敦证券交易所上市,在美国马萨诸塞州、日本东京及香港均设有分公司。
Abcam 一直致力加强产品线,为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的MitoSciences公司,加强了免疫分析方面的产品供应;同年也并购了英国的Ascent Scientific 公司,开展了生化试剂的供应。在2012年,Abcam并购了美国的Epitomics 公司,成为一家有的RabMAbs® 供应商。
Abcam 的 目标是给世界上的抗体建立zui大的在线目录,为各地科学家提供*产品,成为各国科学界的重要伙伴。我们为所有产品提供来使客户获得预期的结 果。为提供高质量的抗体来指向尽可能多的靶蛋白,我们尽所能在尽可能多的应用和物种中检测每种抗体。我们相信诚信才是上策,在我们的上会发布有关我们 每一种产品的尽可能多的信息。
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