InvivoGen pdrive5lc-hfn pDRIVE5Lucia-hFibronectin pDRIVE-Fibronectin
Contents update notification All InvivoGen plasmids are now provided as lyophilized DNA. Specifications: pDRIVE bearing human Fibronectin promoter - LacZ, SEAP or Lucia Gene Promoter: Fibronectin Specificity: Differentiating cells, healing tissues Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia Selection: Zeocin™ Contents: - Provided as 20 µg lyophilized DNA. - 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar). Description: Fibronectin (FN) plays an important role in organizing the extracellular matrix and facilitates cell adhesion, migration, wound healing and tumor metastasis. FN expression is generally low in undifferentiated cells and most adult tissues. However, synthesis is drastically increased after differentiation and during wound healing. Expression of FN is regulated at the transcriptional level in a cell-specific manner by a variety of transcription factors, growth factors and hormones. The FN promoter contains a TATA box, a CAAT sequence, a SP-1 binding site, a cAMP-responsive element [1] and two EGR-1 binding sites. The FN promoter can be activated in vitro in cell lines that normally do not express FN after induction of differentiation [2]. 1. Dean DC. et al. 1987. Cloning and analysis of the promoter region of the human fibronectin gene. Proc Natl Acad Sci USA 84(7): 1876-80. 2. Suzuki M. et al. 1998. Induction of Sp1 in differentiating human embryonal carcinoma cells triggers transcription of the fibronectin gene. Mol Cell Biol. 18(5):3010-20. Details:
ORDERINGpDRIVE-hFibronectinDescription | pDRIVE bearing human Fibronectin promoter - LacZ |
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Cat. Code | pdrive-hfn |
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Unit Size | 20 µg |
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pDRIVE5SEAP-hFibronectinDescription | pDRIVE bearing human Fibronectin promoter - SEAP |
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Cat. Code | pdrive5s-hfn |
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Unit Size | 20 µg |
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pDRIVE5Lucia-hFibronectinDescription | pDRIVE bearing human Fibronectin promoter - Lucia |
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Cat. Code | pdrive5lc-hfn |
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Unit Size | 20 µg |
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