Recombinant Murine IFN-γ

Catalog Number:

315-05

Synonyms:

Immune Interferon, type II interferon, T cell interferon, MAF

IFN-γ是由CD4和CD8 T淋巴细胞，以及活化NK细胞产生的酸不稳定干扰素。的IFN-γ受体存在于大多数免疫细胞，其响应通过增加I类MHC蛋白的表面表达，以IFN-γ信号传导。这促进了抗原与T-辅助（CD4+）细胞的呈现。的IFN-γ的抗原呈递细胞和抗原识别B和T淋巴细胞的信令，调节免疫应答的抗原特异性相。此外，IFN-γ刺激了许多淋巴样细胞的功能，包括巨噬细胞，NK细胞和嗜中性粒细胞的抗微生物和抗肿瘤应答。人IFN-γ是物种特异性的，具有生物活性仅在人类和灵长类动物细胞。重组鼠IFN-γ是含有134个氨基酸残基15.6 kDa的蛋白质。

Description:

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.  Recombinant Murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues.

AA Sequence:

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED₅₀ is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.

Cross Reactivity:

Rabbit, Rat, Mouse, Mouse + Chicken, Human, Cow, N/A, Mouse + Parasite