重庆市华雅干细胞技术有限公司 ，  ：

华雅集团旗下授权代理TTAKARA Cellartis干细胞产品

图片为展示效果，实际产品为hES-MP 002.5细胞株

高矿化潜力的间充质前体细胞

Cellartis-Y10090 hES-MP 002.5人类胚胎干细胞来源的间充质前体细胞

产品英文名称：hES-MP 002.5，Human Multipotent Mesenchymal Progenitor Cells

产品中文名称：人类胚胎干细胞来源的间充质前体细胞

hES-MP 002.5是从人胚胎干细胞诱导分化而得到的间充质干细胞前体细胞（multipotent mesenchymal progenitors）。hES-MP 002.5与人间充质干细胞（human mesenchymal stem， MS）高度相似，可以分化成为多种中胚层细胞种类。hES-MP 002.5由人胚胎干细胞系SA002.5 ES（a subclone of SA002）分化得来，可以向骨细胞进行高效率的分化，分化过程具有着高级别的矿物化潜力（mineralization potential）。hES-MP 002.5细胞产品是高度均匀的、高潜力的间充质干细胞前体细胞，适合于科研级别以及工业规模的间充质干细胞生产。

hES-MP 002.5 cells are human embryonic stem (ES) cell-derived multipotent mesenchymal progenitors that resemble adult human mesenchymal stem (MS) cells and can be differentiated into several mesodermal lineages. The cells are derived from the SA002.5 ES cell line, a subclone of SA002, known for efficient osteogenic lineage differentiation and high mineralization potential with extended culture. hES-MP 002.5 cells are a homogeneous, robust source of mesenchymal progenitors, ideal for bulk production of mesenchymal cells.

产品特点

均质的，批次检测的细胞群; 没有供体到供体的可变问题

细胞强壮易培养

与成人人类间充质干细胞高度相似

能够分化成中胚层谱系

扩增后高矿化潜力High mineralization potential with extended expansion

应用

间充质诱导和分化研究

修复和再生的组织工程模型

Mesenchymal induction and differentiation studies

Tissue engineering models of repair and regeneration

2017应用实例：

研究人员使用Cellartis® hES-MP 002.5细胞表明了流动体可以刺激间充质干细胞的骨基质产生。

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Sasima Puwanun  Robin M. Delaine‐Smith  Helen E. Colley  Julian M. Yates  Sheila MacNeil Gwendolen C. Reilly

Journal of Tissue Engineering and Regenerative Medicine,First published: 9 May 2017 https://doi.org/10.1002/term.2462

组份

1瓶细胞

1 frozen vial of Cellartis® hES-MP 002.5

保存：≤ -150°C。

使用方法

Unpacking of Cellartis® hES-MP 002.5

NOTE! For your protection: Wear a protective face mask and protective gloves. Use forceps when

handling a frozen vial. Never hold the vial in your hand as the cryo vial may explode due to rapid

temperature changes.

NOTE! When transferring the cells from the transport vessel to long time storage, immediate transfer is essential since variations in temperature may have an adverse effect on cell survival and quality.

1. Unpack Cellartis® hES-MP 002.5 as soon as possible after arrival.

2. Check all materials for leakage or breakage.

3. Using forceps immediay place the vial in liquid nitrogen at ≤ -150°C.

自备试剂：

产品                                     建议的制造商            货号

basic Fibroblast Growth Factor (bFGF)            Peprotech              100-18B

Dulbecco’s Modified Eagle Medium (DMEM)    Gibco / Life Technologies   31966-021

胎牛血清（FBS）                          Gibco / Life Technologies   16140

明胶                                     Sigma-Aldrich            G1890

PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS (-/-)) Gibco / Life Technologies    14190

青霉素/链霉素（PEST）                    Gibco / Life Technologies    15140-122

TrypLE Select™                            Gibco / Life Technologies    12563-011

产品性能

Cellartis hES-MP 002.5细胞传30代，细胞群倍加数（cell population doubling level，缩写PDL）没有下降。

Population doubling of human mesenchymal stem cells (hMSCs) and Cellartis hES-MP 002.5 cells through 30 passages. Unlike hMSCs, hES-MP 00.25 cells show no decrease in population doubling level.

订购信息

​华雅集团旗下授权代理TTAKARA Cellartis干细胞产品，Cellartis的多能细胞培养系统包括iPS诱导多能干细胞株、iPS诱导多能干细胞和ES胚胎干细胞培养基，iPS基因编辑用单细胞克隆培养基和多能细胞检测用单克隆抗体。

重庆市华雅干细胞技术有限公司以“专注干细胞研究，提升干细胞品质”为发展理念，专业从事干细胞基础研究、干细胞再生医学临床应用研究及干细胞技术服务，自主研发产品热卖中：人间充质干细胞无血清培养基 

咨询Cellartis-Y10090 Human Multipotent Mesenchymal Progenitor cells ，  ：